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anti pi3k antibody  (Proteintech)


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    Structured Review

    Proteintech anti pi3k antibody
    Anti Pi3k Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 317 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+pi3k/PI3K+p85+alpha+Antibody/pmc13620287-191-75-77
    Average 96 stars, based on 317 article reviews
    anti pi3k antibody - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: TRIM21-Mediated ubiquitination of FBL suppresses PI3K/AKT signaling and tumor progression in clear cell renal cell carcinoma.
    Article Snippet: .. Membranes were blocked with 5% nonfat milk for 1 h at room temperature and incubated overnight at 4 ° C with the following primary antibodies: anti-FBL (Proteintech, Cat# 66985-1-Ig), anti-Trim21 (Proteintech, Cat# 12108-1-AP), anti-PI3K (Proteintech, Cat# 20584-1-AP), anti-p-AKT (Ser473; Proteintech, Cat# 66444-1-Ig), anti-AKT (Proteintech, Cat# 10176-2-AP), anti-HA tag (Proteintech, Cat# 66006-2-Ig), anti-FLAG tag (Proteintech, Cat# 20543-1- AP), anti-CREB (Proteintech, Cat# 12208-1-AP), anti-p-CREB (Ser133; Proteintech, Cat# 28792-1-AP) and anti-ubiquitin (UB; Jingjie PTM BioLab, Cat# PTM-7311). .. β -actin (Proteintech, Cat# 66009-1-Ig) was used as a loading control.

    Article Title: Growth differentiation factor 15 promotes the malignant progression of multiple myeloma via activation of PI3K/Akt/NF-κB signaling pathway
    Article Snippet: .. Membranes were blocked with QuickBlockTM Western Blocking Reagent (P0252, Beyotime, Foshan, Guangdong, China) for 15 min at room temperature, followed by incubation with the following primary antibodies at 4 °C overnight: anti-E-cadherin (1:1000; 28731-1-AP), anti-CXCR4 (1:1000; 11073-2-AP), anti-Akt (1:1000; 10176-2-AP), anti-p-Akt (1:1000; 28731-1-AP), anti-MMP9 (1:1000; 10375-2-AP), anti-GAPDH (1:1000; 10494-1-AP), anti-BAX (1:1000; 50599-2-Ig), anti-BCL-2 (1:1000; 12789-1-AP), anti-caspase-9 (1:1000; 10380-1-AP), anti-PI3K (1:1000; 67121-1-Ig) (all from Proteintech, Wuhan, Hubei, China), anti-NF-κB p65 (1:1000; WL01273b, Wanleibio, Shenyang, Liaoning, China), and anti-GFRAL (1:1500; CSB-PA751020LA01HU, CUSABIO, Wuhan, Hubei, China). .. Following primary antibody incubation, membranes were incubated with horseradish peroxidase-conjugated AffiniPure goat anti-rabbit IgG secondary antibody (1:6000; BA1054; Boster Bio, Wuhan, Hubei, China) for 2 h at room temperature.

    Article Title: TRIM21-Mediated ubiquitination of FBL suppresses PI3K/AKT signaling and tumor progression in clear cell renal cell carcinoma
    Article Snippet: .. Membranes were blocked with 5% nonfat milk for 1 h at room temperature and incubated overnight at 4 °C with the following primary antibodies: anti-FBL (Proteintech, Cat# 66985-1-Ig), anti-Trim21 (Proteintech, Cat# 12108-1-AP), anti-PI3K (Proteintech, Cat# 20584-1-AP), anti-p-AKT (Ser473; Proteintech, Cat# 66444-1-Ig), anti-AKT (Proteintech, Cat# 10176-2-AP), anti-HA tag (Proteintech, Cat# 66006-2-Ig), anti-FLAG tag (Proteintech, Cat# 20543-1-AP), anti-CREB (Proteintech, Cat# 12208-1-AP), anti-p-CREB (Ser133; Proteintech, Cat# 28792-1-AP) and anti-ubiquitin (UB; Jingjie PTM BioLab, Cat# PTM-7311). .. β-actin (Proteintech, Cat# 66009-1-Ig) was used as a loading control.

    Article Title: Growth differentiation factor 15 promotes the malignant progression of multiple myeloma via activation of PI3K/Akt/NF-κB signaling pathway.
    Article Snippet: .. Membranes were blocked with QuickBlockTM Western Blocking Reagent (P0252, Beyotime, Foshan, Guangdong, China) for 15 minutes at room temperature, followed by incubation with the following primary antibodies at 4 °C overnight: anti-E-cadherin (1:1000; 28731-1-AP), anti-CXCR4 (1:1000; 11073-2-AP), anti-Akt (1:1000; 10176-2-AP), anti-p-Akt (1:1000; 28731- 1-AP), anti-MMP9 (1:1000; 10375-2-AP), anti-GAPDH (1:1000; 10494-1-AP), anti-BAX (1:1000; 50599-2-Ig), antiBCL-2 (1:1000; 12789-1-AP), anti-caspase-9 (1:1000; 10380-1-AP), anti-PI3K (1:1000; 67121-1-Ig) (all from Proteintech, Wuhan, Hubei, China), anti-NF-κB p65 AR TIC LE IN PR ES S (1:1000; WL01273b, Wanleibio, Shenyang, Liaoning, China), and anti-GFRAL (1:1500; CSB-PA751020LA01HU, CUSABIO, Wuhan, Hubei, China). .. Total proteins were extracted using a total protein extraction kit (EX1100, Solarbio, Beijing, China) and quantified with a BCA protein assay kit (MA0082, Meilunbio, Dalian, Liaoning, China) according to the manufacturer’s instructions.

    Western Blot:

    Article Title: Growth differentiation factor 15 promotes the malignant progression of multiple myeloma via activation of PI3K/Akt/NF-κB signaling pathway
    Article Snippet: .. Membranes were blocked with QuickBlockTM Western Blocking Reagent (P0252, Beyotime, Foshan, Guangdong, China) for 15 min at room temperature, followed by incubation with the following primary antibodies at 4 °C overnight: anti-E-cadherin (1:1000; 28731-1-AP), anti-CXCR4 (1:1000; 11073-2-AP), anti-Akt (1:1000; 10176-2-AP), anti-p-Akt (1:1000; 28731-1-AP), anti-MMP9 (1:1000; 10375-2-AP), anti-GAPDH (1:1000; 10494-1-AP), anti-BAX (1:1000; 50599-2-Ig), anti-BCL-2 (1:1000; 12789-1-AP), anti-caspase-9 (1:1000; 10380-1-AP), anti-PI3K (1:1000; 67121-1-Ig) (all from Proteintech, Wuhan, Hubei, China), anti-NF-κB p65 (1:1000; WL01273b, Wanleibio, Shenyang, Liaoning, China), and anti-GFRAL (1:1500; CSB-PA751020LA01HU, CUSABIO, Wuhan, Hubei, China). .. Following primary antibody incubation, membranes were incubated with horseradish peroxidase-conjugated AffiniPure goat anti-rabbit IgG secondary antibody (1:6000; BA1054; Boster Bio, Wuhan, Hubei, China) for 2 h at room temperature.

    Article Title: Growth differentiation factor 15 promotes the malignant progression of multiple myeloma via activation of PI3K/Akt/NF-κB signaling pathway.
    Article Snippet: .. Membranes were blocked with QuickBlockTM Western Blocking Reagent (P0252, Beyotime, Foshan, Guangdong, China) for 15 minutes at room temperature, followed by incubation with the following primary antibodies at 4 °C overnight: anti-E-cadherin (1:1000; 28731-1-AP), anti-CXCR4 (1:1000; 11073-2-AP), anti-Akt (1:1000; 10176-2-AP), anti-p-Akt (1:1000; 28731- 1-AP), anti-MMP9 (1:1000; 10375-2-AP), anti-GAPDH (1:1000; 10494-1-AP), anti-BAX (1:1000; 50599-2-Ig), antiBCL-2 (1:1000; 12789-1-AP), anti-caspase-9 (1:1000; 10380-1-AP), anti-PI3K (1:1000; 67121-1-Ig) (all from Proteintech, Wuhan, Hubei, China), anti-NF-κB p65 AR TIC LE IN PR ES S (1:1000; WL01273b, Wanleibio, Shenyang, Liaoning, China), and anti-GFRAL (1:1500; CSB-PA751020LA01HU, CUSABIO, Wuhan, Hubei, China). .. Total proteins were extracted using a total protein extraction kit (EX1100, Solarbio, Beijing, China) and quantified with a BCA protein assay kit (MA0082, Meilunbio, Dalian, Liaoning, China) according to the manufacturer’s instructions.

    Blocking Assay:

    Article Title: Growth differentiation factor 15 promotes the malignant progression of multiple myeloma via activation of PI3K/Akt/NF-κB signaling pathway
    Article Snippet: .. Membranes were blocked with QuickBlockTM Western Blocking Reagent (P0252, Beyotime, Foshan, Guangdong, China) for 15 min at room temperature, followed by incubation with the following primary antibodies at 4 °C overnight: anti-E-cadherin (1:1000; 28731-1-AP), anti-CXCR4 (1:1000; 11073-2-AP), anti-Akt (1:1000; 10176-2-AP), anti-p-Akt (1:1000; 28731-1-AP), anti-MMP9 (1:1000; 10375-2-AP), anti-GAPDH (1:1000; 10494-1-AP), anti-BAX (1:1000; 50599-2-Ig), anti-BCL-2 (1:1000; 12789-1-AP), anti-caspase-9 (1:1000; 10380-1-AP), anti-PI3K (1:1000; 67121-1-Ig) (all from Proteintech, Wuhan, Hubei, China), anti-NF-κB p65 (1:1000; WL01273b, Wanleibio, Shenyang, Liaoning, China), and anti-GFRAL (1:1500; CSB-PA751020LA01HU, CUSABIO, Wuhan, Hubei, China). .. Following primary antibody incubation, membranes were incubated with horseradish peroxidase-conjugated AffiniPure goat anti-rabbit IgG secondary antibody (1:6000; BA1054; Boster Bio, Wuhan, Hubei, China) for 2 h at room temperature.

    Article Title: Growth differentiation factor 15 promotes the malignant progression of multiple myeloma via activation of PI3K/Akt/NF-κB signaling pathway.
    Article Snippet: .. Membranes were blocked with QuickBlockTM Western Blocking Reagent (P0252, Beyotime, Foshan, Guangdong, China) for 15 minutes at room temperature, followed by incubation with the following primary antibodies at 4 °C overnight: anti-E-cadherin (1:1000; 28731-1-AP), anti-CXCR4 (1:1000; 11073-2-AP), anti-Akt (1:1000; 10176-2-AP), anti-p-Akt (1:1000; 28731- 1-AP), anti-MMP9 (1:1000; 10375-2-AP), anti-GAPDH (1:1000; 10494-1-AP), anti-BAX (1:1000; 50599-2-Ig), antiBCL-2 (1:1000; 12789-1-AP), anti-caspase-9 (1:1000; 10380-1-AP), anti-PI3K (1:1000; 67121-1-Ig) (all from Proteintech, Wuhan, Hubei, China), anti-NF-κB p65 AR TIC LE IN PR ES S (1:1000; WL01273b, Wanleibio, Shenyang, Liaoning, China), and anti-GFRAL (1:1500; CSB-PA751020LA01HU, CUSABIO, Wuhan, Hubei, China). .. Total proteins were extracted using a total protein extraction kit (EX1100, Solarbio, Beijing, China) and quantified with a BCA protein assay kit (MA0082, Meilunbio, Dalian, Liaoning, China) according to the manufacturer’s instructions.



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    Image Search Results


    Effects of LY294002 and PTD-FNK on HS-Induced SCs. ( A ) LY294002 Dose-Dependent Viability Profiling (0, 1, 2, 5, 10 and 20 μM); ( B ) Effects of LY294002 on ROS levels in HS-induced SCs; LY294002 Modulates Apoptotic Mediators: Bax ( C ), Bcl-2 ( D ), Caspase-3 ( E ), and Cyt C ( F ); ( G ) Effect of LY294002 on Bax, Bcl-2, p-PI3K, PI3K, p-Akt and Akt protein expression in HS-Induced SCs. (ns, not significant, * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001) (the original Western blot pictures can be found in ).

    Journal: Veterinary Sciences

    Article Title: PTD-FNK Alleviated Heat Stress-Induced Apoptosis of Boar Sertoli Cells via the PI3K/AKT Pathway

    doi: 10.3390/vetsci13080798

    Figure Lengend Snippet: Effects of LY294002 and PTD-FNK on HS-Induced SCs. ( A ) LY294002 Dose-Dependent Viability Profiling (0, 1, 2, 5, 10 and 20 μM); ( B ) Effects of LY294002 on ROS levels in HS-induced SCs; LY294002 Modulates Apoptotic Mediators: Bax ( C ), Bcl-2 ( D ), Caspase-3 ( E ), and Cyt C ( F ); ( G ) Effect of LY294002 on Bax, Bcl-2, p-PI3K, PI3K, p-Akt and Akt protein expression in HS-Induced SCs. (ns, not significant, * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001) (the original Western blot pictures can be found in ).

    Article Snippet: The membranes were blocked with 5% non-fat milk for 1 h at room temperature, then incubated overnight at 4 °C with a primary antibody against Bax (1:1000; CST, Danvers, MA, USA), Bcl-2 (1:1000; HUABIO, Hangzhou, China), P-PI3K (1:1000; CST, Danvers, MA, USA), PI3K (1:1000; ABclonal, Wuhan, China), P-AKT (1:1000; ABclonal), AKT (1:1000; Abcam, Cambridge, UK), and actin (1:1000; CST, Danvers, MA, USA,).

    Techniques: Expressing, Western Blot