Journal: Veterinary Sciences

Article Title: PTD-FNK Alleviated Heat Stress-Induced Apoptosis of Boar Sertoli Cells via the PI3K/AKT Pathway

doi: 10.3390/vetsci13080798

Figure Lengend Snippet: Effects of LY294002 and PTD-FNK on HS-Induced SCs. ( A ) LY294002 Dose-Dependent Viability Profiling (0, 1, 2, 5, 10 and 20 μM); ( B ) Effects of LY294002 on ROS levels in HS-induced SCs; LY294002 Modulates Apoptotic Mediators: Bax ( C ), Bcl-2 ( D ), Caspase-3 ( E ), and Cyt C ( F ); ( G ) Effect of LY294002 on Bax, Bcl-2, p-PI3K, PI3K, p-Akt and Akt protein expression in HS-Induced SCs. (ns, not significant, * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001) (the original Western blot pictures can be found in ).

Article Snippet: The membranes were blocked with 5% non-fat milk for 1 h at room temperature, then incubated overnight at 4 °C with a primary antibody against Bax (1:1000; CST, Danvers, MA, USA), Bcl-2 (1:1000; HUABIO, Hangzhou, China), P-PI3K (1:1000; CST, Danvers, MA, USA), PI3K (1:1000; ABclonal, Wuhan, China), P-AKT (1:1000; ABclonal), AKT (1:1000; Abcam, Cambridge, UK), and actin (1:1000; CST, Danvers, MA, USA,).

Techniques: Expressing, Western Blot

Journal: Poultry Science

Article Title: Pathogenic mechanism of Eimeria tenella Et MIC2 promotes Eimeria tenella invasion and inhibits host cell apoptosis through binding to the ITGAV receptor

doi: 10.1016/j.psj.2026.106922

Figure Lengend Snippet: The mRNA expression of ITGAV, FAK, PLC, PKC, p65, ERK, JNK, p38, PI3K, Akt, Bax, Bcl2 , and Caspase 3 in E.tenella host cells.

Article Snippet: p-PI3K Rabbit Ab , Abmart , P76365R4 , 1: 1500.

Techniques: Expressing

Journal: Poultry Science

Article Title: Pathogenic mechanism of Eimeria tenella Et MIC2 promotes Eimeria tenella invasion and inhibits host cell apoptosis through binding to the ITGAV receptor

doi: 10.1016/j.psj.2026.106922

Figure Lengend Snippet: The protein activity changes of ITGAV, FAK, PLC, PKC, p65, ERK, JNK, p38, PI3K, Akt, Bax, Bcl2, and Caspase 3 in E.tenella host cells.

Article Snippet: p-PI3K Rabbit Ab , Abmart , P76365R4 , 1: 1500.

Techniques: Activity Assay

Journal: Poultry Science

Article Title: Pathogenic mechanism of Eimeria tenella Et MIC2 promotes Eimeria tenella invasion and inhibits host cell apoptosis through binding to the ITGAV receptor

doi: 10.1016/j.psj.2026.106922

Figure Lengend Snippet: The mRNA expression of ITGAV, FAK, PLC, PKC, p65, ERK, JNK, p38, PI3K, Akt, Bax, Bcl2 , and Caspase 3 in E.tenella host cells.

Article Snippet: Membranes were blocked with 5 % BSA at 37°C for 2 h and incubated overnight at 4°C with the following primary antibodies: ITGAV Rabbit Ab, PLC Rabbit Ab, p-PLC Rabbit Ab, p-p65 Rabbit Ab, and Bcl2 Rabbit Ab were purchased from Bioss (Beijing, China); FAK Rabbit Ab, p-FAK Rabbit Ab, ERK Rabbit Ab, p-ERK Rabbit Ab, JNK Rabbit Ab, p-JNK Rabbit Ab, p38 MAPK Rabbit Ab, p-p38 MAPK Rabbit Ab, PI3K Rabbit Ab, p-PI3K Rabbit Ab, AKT Rabbit Ab, p-AKT Rabbit Ab, PKC Rabbit Ab, p-PKC Rabbit Ab, Bax Rabbit Ab, and Caspase 3 Rabbit Ab were purchased from Abmart (Shanghai, China); p65 Rabbit Ab (Proteintech, Wuhan, China).

Techniques: Expressing

Journal: Poultry Science

Article Title: Pathogenic mechanism of Eimeria tenella Et MIC2 promotes Eimeria tenella invasion and inhibits host cell apoptosis through binding to the ITGAV receptor

doi: 10.1016/j.psj.2026.106922

Figure Lengend Snippet: The protein activity changes of ITGAV, FAK, PLC, PKC, p65, ERK, JNK, p38, PI3K, Akt, Bax, Bcl2, and Caspase 3 in E.tenella host cells.

Article Snippet: Membranes were blocked with 5 % BSA at 37°C for 2 h and incubated overnight at 4°C with the following primary antibodies: ITGAV Rabbit Ab, PLC Rabbit Ab, p-PLC Rabbit Ab, p-p65 Rabbit Ab, and Bcl2 Rabbit Ab were purchased from Bioss (Beijing, China); FAK Rabbit Ab, p-FAK Rabbit Ab, ERK Rabbit Ab, p-ERK Rabbit Ab, JNK Rabbit Ab, p-JNK Rabbit Ab, p38 MAPK Rabbit Ab, p-p38 MAPK Rabbit Ab, PI3K Rabbit Ab, p-PI3K Rabbit Ab, AKT Rabbit Ab, p-AKT Rabbit Ab, PKC Rabbit Ab, p-PKC Rabbit Ab, Bax Rabbit Ab, and Caspase 3 Rabbit Ab were purchased from Abmart (Shanghai, China); p65 Rabbit Ab (Proteintech, Wuhan, China).

Techniques: Activity Assay