Review




Structured Review

Proteintech anti pi3k
Anti Pi3k, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 163 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+pi3k/PI3K+p110(alpha)+Antibody/pmc13658529-115-27-28
Average 95 stars, based on 163 article reviews
anti pi3k - by Bioz Stars, 2026-10
95/100 stars

Images

Related Articles

Incubation:

Article Title: TRIM21-Mediated ubiquitination of FBL suppresses PI3K/AKT signaling and tumor progression in clear cell renal cell carcinoma.
Article Snippet: .. Membranes were blocked with 5% nonfat milk for 1 h at room temperature and incubated overnight at 4 ° C with the following primary antibodies: anti-FBL (Proteintech, Cat# 66985-1-Ig), anti-Trim21 (Proteintech, Cat# 12108-1-AP), anti-PI3K (Proteintech, Cat# 20584-1-AP), anti-p-AKT (Ser473; Proteintech, Cat# 66444-1-Ig), anti-AKT (Proteintech, Cat# 10176-2-AP), anti-HA tag (Proteintech, Cat# 66006-2-Ig), anti-FLAG tag (Proteintech, Cat# 20543-1- AP), anti-CREB (Proteintech, Cat# 12208-1-AP), anti-p-CREB (Ser133; Proteintech, Cat# 28792-1-AP) and anti-ubiquitin (UB; Jingjie PTM BioLab, Cat# PTM-7311). .. β -actin (Proteintech, Cat# 66009-1-Ig) was used as a loading control.

Article Title: Growth differentiation factor 15 promotes the malignant progression of multiple myeloma via activation of PI3K/Akt/NF-κB signaling pathway
Article Snippet: .. Membranes were blocked with QuickBlockTM Western Blocking Reagent (P0252, Beyotime, Foshan, Guangdong, China) for 15 min at room temperature, followed by incubation with the following primary antibodies at 4 °C overnight: anti-E-cadherin (1:1000; 28731-1-AP), anti-CXCR4 (1:1000; 11073-2-AP), anti-Akt (1:1000; 10176-2-AP), anti-p-Akt (1:1000; 28731-1-AP), anti-MMP9 (1:1000; 10375-2-AP), anti-GAPDH (1:1000; 10494-1-AP), anti-BAX (1:1000; 50599-2-Ig), anti-BCL-2 (1:1000; 12789-1-AP), anti-caspase-9 (1:1000; 10380-1-AP), anti-PI3K (1:1000; 67121-1-Ig) (all from Proteintech, Wuhan, Hubei, China), anti-NF-κB p65 (1:1000; WL01273b, Wanleibio, Shenyang, Liaoning, China), and anti-GFRAL (1:1500; CSB-PA751020LA01HU, CUSABIO, Wuhan, Hubei, China). .. Following primary antibody incubation, membranes were incubated with horseradish peroxidase-conjugated AffiniPure goat anti-rabbit IgG secondary antibody (1:6000; BA1054; Boster Bio, Wuhan, Hubei, China) for 2 h at room temperature.

Article Title: TRIM21-Mediated ubiquitination of FBL suppresses PI3K/AKT signaling and tumor progression in clear cell renal cell carcinoma
Article Snippet: .. Membranes were blocked with 5% nonfat milk for 1 h at room temperature and incubated overnight at 4 °C with the following primary antibodies: anti-FBL (Proteintech, Cat# 66985-1-Ig), anti-Trim21 (Proteintech, Cat# 12108-1-AP), anti-PI3K (Proteintech, Cat# 20584-1-AP), anti-p-AKT (Ser473; Proteintech, Cat# 66444-1-Ig), anti-AKT (Proteintech, Cat# 10176-2-AP), anti-HA tag (Proteintech, Cat# 66006-2-Ig), anti-FLAG tag (Proteintech, Cat# 20543-1-AP), anti-CREB (Proteintech, Cat# 12208-1-AP), anti-p-CREB (Ser133; Proteintech, Cat# 28792-1-AP) and anti-ubiquitin (UB; Jingjie PTM BioLab, Cat# PTM-7311). .. β-actin (Proteintech, Cat# 66009-1-Ig) was used as a loading control.

Article Title: Growth differentiation factor 15 promotes the malignant progression of multiple myeloma via activation of PI3K/Akt/NF-κB signaling pathway.
Article Snippet: .. Membranes were blocked with QuickBlockTM Western Blocking Reagent (P0252, Beyotime, Foshan, Guangdong, China) for 15 minutes at room temperature, followed by incubation with the following primary antibodies at 4 °C overnight: anti-E-cadherin (1:1000; 28731-1-AP), anti-CXCR4 (1:1000; 11073-2-AP), anti-Akt (1:1000; 10176-2-AP), anti-p-Akt (1:1000; 28731- 1-AP), anti-MMP9 (1:1000; 10375-2-AP), anti-GAPDH (1:1000; 10494-1-AP), anti-BAX (1:1000; 50599-2-Ig), antiBCL-2 (1:1000; 12789-1-AP), anti-caspase-9 (1:1000; 10380-1-AP), anti-PI3K (1:1000; 67121-1-Ig) (all from Proteintech, Wuhan, Hubei, China), anti-NF-κB p65 AR TIC LE IN PR ES S (1:1000; WL01273b, Wanleibio, Shenyang, Liaoning, China), and anti-GFRAL (1:1500; CSB-PA751020LA01HU, CUSABIO, Wuhan, Hubei, China). .. Total proteins were extracted using a total protein extraction kit (EX1100, Solarbio, Beijing, China) and quantified with a BCA protein assay kit (MA0082, Meilunbio, Dalian, Liaoning, China) according to the manufacturer’s instructions.

Western Blot:

Article Title: Growth differentiation factor 15 promotes the malignant progression of multiple myeloma via activation of PI3K/Akt/NF-κB signaling pathway
Article Snippet: .. Membranes were blocked with QuickBlockTM Western Blocking Reagent (P0252, Beyotime, Foshan, Guangdong, China) for 15 min at room temperature, followed by incubation with the following primary antibodies at 4 °C overnight: anti-E-cadherin (1:1000; 28731-1-AP), anti-CXCR4 (1:1000; 11073-2-AP), anti-Akt (1:1000; 10176-2-AP), anti-p-Akt (1:1000; 28731-1-AP), anti-MMP9 (1:1000; 10375-2-AP), anti-GAPDH (1:1000; 10494-1-AP), anti-BAX (1:1000; 50599-2-Ig), anti-BCL-2 (1:1000; 12789-1-AP), anti-caspase-9 (1:1000; 10380-1-AP), anti-PI3K (1:1000; 67121-1-Ig) (all from Proteintech, Wuhan, Hubei, China), anti-NF-κB p65 (1:1000; WL01273b, Wanleibio, Shenyang, Liaoning, China), and anti-GFRAL (1:1500; CSB-PA751020LA01HU, CUSABIO, Wuhan, Hubei, China). .. Following primary antibody incubation, membranes were incubated with horseradish peroxidase-conjugated AffiniPure goat anti-rabbit IgG secondary antibody (1:6000; BA1054; Boster Bio, Wuhan, Hubei, China) for 2 h at room temperature.

Article Title: Growth differentiation factor 15 promotes the malignant progression of multiple myeloma via activation of PI3K/Akt/NF-κB signaling pathway.
Article Snippet: .. Membranes were blocked with QuickBlockTM Western Blocking Reagent (P0252, Beyotime, Foshan, Guangdong, China) for 15 minutes at room temperature, followed by incubation with the following primary antibodies at 4 °C overnight: anti-E-cadherin (1:1000; 28731-1-AP), anti-CXCR4 (1:1000; 11073-2-AP), anti-Akt (1:1000; 10176-2-AP), anti-p-Akt (1:1000; 28731- 1-AP), anti-MMP9 (1:1000; 10375-2-AP), anti-GAPDH (1:1000; 10494-1-AP), anti-BAX (1:1000; 50599-2-Ig), antiBCL-2 (1:1000; 12789-1-AP), anti-caspase-9 (1:1000; 10380-1-AP), anti-PI3K (1:1000; 67121-1-Ig) (all from Proteintech, Wuhan, Hubei, China), anti-NF-κB p65 AR TIC LE IN PR ES S (1:1000; WL01273b, Wanleibio, Shenyang, Liaoning, China), and anti-GFRAL (1:1500; CSB-PA751020LA01HU, CUSABIO, Wuhan, Hubei, China). .. Total proteins were extracted using a total protein extraction kit (EX1100, Solarbio, Beijing, China) and quantified with a BCA protein assay kit (MA0082, Meilunbio, Dalian, Liaoning, China) according to the manufacturer’s instructions.

Blocking Assay:

Article Title: Growth differentiation factor 15 promotes the malignant progression of multiple myeloma via activation of PI3K/Akt/NF-κB signaling pathway
Article Snippet: .. Membranes were blocked with QuickBlockTM Western Blocking Reagent (P0252, Beyotime, Foshan, Guangdong, China) for 15 min at room temperature, followed by incubation with the following primary antibodies at 4 °C overnight: anti-E-cadherin (1:1000; 28731-1-AP), anti-CXCR4 (1:1000; 11073-2-AP), anti-Akt (1:1000; 10176-2-AP), anti-p-Akt (1:1000; 28731-1-AP), anti-MMP9 (1:1000; 10375-2-AP), anti-GAPDH (1:1000; 10494-1-AP), anti-BAX (1:1000; 50599-2-Ig), anti-BCL-2 (1:1000; 12789-1-AP), anti-caspase-9 (1:1000; 10380-1-AP), anti-PI3K (1:1000; 67121-1-Ig) (all from Proteintech, Wuhan, Hubei, China), anti-NF-κB p65 (1:1000; WL01273b, Wanleibio, Shenyang, Liaoning, China), and anti-GFRAL (1:1500; CSB-PA751020LA01HU, CUSABIO, Wuhan, Hubei, China). .. Following primary antibody incubation, membranes were incubated with horseradish peroxidase-conjugated AffiniPure goat anti-rabbit IgG secondary antibody (1:6000; BA1054; Boster Bio, Wuhan, Hubei, China) for 2 h at room temperature.

Article Title: Growth differentiation factor 15 promotes the malignant progression of multiple myeloma via activation of PI3K/Akt/NF-κB signaling pathway.
Article Snippet: .. Membranes were blocked with QuickBlockTM Western Blocking Reagent (P0252, Beyotime, Foshan, Guangdong, China) for 15 minutes at room temperature, followed by incubation with the following primary antibodies at 4 °C overnight: anti-E-cadherin (1:1000; 28731-1-AP), anti-CXCR4 (1:1000; 11073-2-AP), anti-Akt (1:1000; 10176-2-AP), anti-p-Akt (1:1000; 28731- 1-AP), anti-MMP9 (1:1000; 10375-2-AP), anti-GAPDH (1:1000; 10494-1-AP), anti-BAX (1:1000; 50599-2-Ig), antiBCL-2 (1:1000; 12789-1-AP), anti-caspase-9 (1:1000; 10380-1-AP), anti-PI3K (1:1000; 67121-1-Ig) (all from Proteintech, Wuhan, Hubei, China), anti-NF-κB p65 AR TIC LE IN PR ES S (1:1000; WL01273b, Wanleibio, Shenyang, Liaoning, China), and anti-GFRAL (1:1500; CSB-PA751020LA01HU, CUSABIO, Wuhan, Hubei, China). .. Total proteins were extracted using a total protein extraction kit (EX1100, Solarbio, Beijing, China) and quantified with a BCA protein assay kit (MA0082, Meilunbio, Dalian, Liaoning, China) according to the manufacturer’s instructions.



Similar Products

96
Cell Signaling Technology Inc phospho pi3k p85 tyr458
ARHGAP40 associates with RhoA and is accompanied by reduced RhoA activity, <t>PI3K/AKT</t> signaling, and altered glutamine-related/redox measures. (A) Flag-based co-immunoprecipitation showing association of ARHGAP40 with RhoA. (B) Endogenous immunoprecipitation of RhoA followed by immunoblotting for ARHGAP40. (C) RhoA activity assay using a conformation-specific anti-RhoA-GTP antibody, with representative GTP-RhoA and total RhoA blots and quantification of the GTP-RhoA/total RhoA ratio. (D) Representative western blots and densitometric analyzes of p -AKT/AKT and p -PI3K/PI3K. (E) Intracellular glutamate levels. (F) Intracellular glutamine levels. (G) GSH/GSSG ratios. (H) Representative ROS flow-cytometry histograms and mean fluorescence intensity. Quantitative analyzes used n = 3 independent biological replicates and are shown as mean ± SEM. Two-group comparisons were performed using two-tailed Student's t-test. * P < 0.05, ** P < 0.01, *** P < 0.001.
Phospho Pi3k P85 Tyr458, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+pi3k/Phospho-PI3+Kinase+p85+(Tyr458)%2Fp55+(Tyr199)+Antibody/pmc13652080-111-41-44
Average 96 stars, based on 1 article reviews
phospho pi3k p85 tyr458 - by Bioz Stars, 2026-10
96/100 stars
  Buy from Supplier

96
Cell Signaling Technology Inc p pi3k
ARHGAP40 associates with RhoA and is accompanied by reduced RhoA activity, <t>PI3K/AKT</t> signaling, and altered glutamine-related/redox measures. (A) Flag-based co-immunoprecipitation showing association of ARHGAP40 with RhoA. (B) Endogenous immunoprecipitation of RhoA followed by immunoblotting for ARHGAP40. (C) RhoA activity assay using a conformation-specific anti-RhoA-GTP antibody, with representative GTP-RhoA and total RhoA blots and quantification of the GTP-RhoA/total RhoA ratio. (D) Representative western blots and densitometric analyzes of p -AKT/AKT and p -PI3K/PI3K. (E) Intracellular glutamate levels. (F) Intracellular glutamine levels. (G) GSH/GSSG ratios. (H) Representative ROS flow-cytometry histograms and mean fluorescence intensity. Quantitative analyzes used n = 3 independent biological replicates and are shown as mean ± SEM. Two-group comparisons were performed using two-tailed Student's t-test. * P < 0.05, ** P < 0.01, *** P < 0.001.
P Pi3k, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+pi3k/Phospho-PI3+Kinase+p85+(Tyr458)%2Fp55+(Tyr199)+Antibody/pm42834668-486-26-28
Average 96 stars, based on 1 article reviews
p pi3k - by Bioz Stars, 2026-10
96/100 stars
  Buy from Supplier

96
Cell Signaling Technology Inc antibodies against pi3k
ARHGAP40 associates with RhoA and is accompanied by reduced RhoA activity, <t>PI3K/AKT</t> signaling, and altered glutamine-related/redox measures. (A) Flag-based co-immunoprecipitation showing association of ARHGAP40 with RhoA. (B) Endogenous immunoprecipitation of RhoA followed by immunoblotting for ARHGAP40. (C) RhoA activity assay using a conformation-specific anti-RhoA-GTP antibody, with representative GTP-RhoA and total RhoA blots and quantification of the GTP-RhoA/total RhoA ratio. (D) Representative western blots and densitometric analyzes of p -AKT/AKT and p -PI3K/PI3K. (E) Intracellular glutamate levels. (F) Intracellular glutamine levels. (G) GSH/GSSG ratios. (H) Representative ROS flow-cytometry histograms and mean fluorescence intensity. Quantitative analyzes used n = 3 independent biological replicates and are shown as mean ± SEM. Two-group comparisons were performed using two-tailed Student's t-test. * P < 0.05, ** P < 0.01, *** P < 0.001.
Antibodies Against Pi3k, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+pi3k/PI3+Kinase+p85+Rabbit+mAb/pm42834668-486-20-25
Average 96 stars, based on 1 article reviews
antibodies against pi3k - by Bioz Stars, 2026-10
96/100 stars
  Buy from Supplier

pi3k  (Bioss)
94
Bioss pi3k
ARHGAP40 associates with RhoA and is accompanied by reduced RhoA activity, <t>PI3K/AKT</t> signaling, and altered glutamine-related/redox measures. (A) Flag-based co-immunoprecipitation showing association of ARHGAP40 with RhoA. (B) Endogenous immunoprecipitation of RhoA followed by immunoblotting for ARHGAP40. (C) RhoA activity assay using a conformation-specific anti-RhoA-GTP antibody, with representative GTP-RhoA and total RhoA blots and quantification of the GTP-RhoA/total RhoA ratio. (D) Representative western blots and densitometric analyzes of p -AKT/AKT and p -PI3K/PI3K. (E) Intracellular glutamate levels. (F) Intracellular glutamine levels. (G) GSH/GSSG ratios. (H) Representative ROS flow-cytometry histograms and mean fluorescence intensity. Quantitative analyzes used n = 3 independent biological replicates and are shown as mean ± SEM. Two-group comparisons were performed using two-tailed Student's t-test. * P < 0.05, ** P < 0.01, *** P < 0.001.
Pi3k, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+pi3k/PI3+kinase+p85+alpha+subunit+Antibody/pm42822614-163-42-43
Average 94 stars, based on 1 article reviews
pi3k - by Bioz Stars, 2026-10
94/100 stars
  Buy from Supplier

94
MedChemExpress rabbit anti p pi3k
ARHGAP40 associates with RhoA and is accompanied by reduced RhoA activity, <t>PI3K/AKT</t> signaling, and altered glutamine-related/redox measures. (A) Flag-based co-immunoprecipitation showing association of ARHGAP40 with RhoA. (B) Endogenous immunoprecipitation of RhoA followed by immunoblotting for ARHGAP40. (C) RhoA activity assay using a conformation-specific anti-RhoA-GTP antibody, with representative GTP-RhoA and total RhoA blots and quantification of the GTP-RhoA/total RhoA ratio. (D) Representative western blots and densitometric analyzes of p -AKT/AKT and p -PI3K/PI3K. (E) Intracellular glutamate levels. (F) Intracellular glutamine levels. (G) GSH/GSSG ratios. (H) Representative ROS flow-cytometry histograms and mean fluorescence intensity. Quantitative analyzes used n = 3 independent biological replicates and are shown as mean ± SEM. Two-group comparisons were performed using two-tailed Student's t-test. * P < 0.05, ** P < 0.01, *** P < 0.001.
Rabbit Anti P Pi3k, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+pi3k/Phospho-PI3+Kinase+p85%2Fp55+(Tyr467%2FTyr199)+Antibody/10__1016_slash_j__prp__2026__156707-142-96-100
Average 94 stars, based on 1 article reviews
rabbit anti p pi3k - by Bioz Stars, 2026-10
94/100 stars
  Buy from Supplier

95
Proteintech anti pi3k
ARHGAP40 associates with RhoA and is accompanied by reduced RhoA activity, <t>PI3K/AKT</t> signaling, and altered glutamine-related/redox measures. (A) Flag-based co-immunoprecipitation showing association of ARHGAP40 with RhoA. (B) Endogenous immunoprecipitation of RhoA followed by immunoblotting for ARHGAP40. (C) RhoA activity assay using a conformation-specific anti-RhoA-GTP antibody, with representative GTP-RhoA and total RhoA blots and quantification of the GTP-RhoA/total RhoA ratio. (D) Representative western blots and densitometric analyzes of p -AKT/AKT and p -PI3K/PI3K. (E) Intracellular glutamate levels. (F) Intracellular glutamine levels. (G) GSH/GSSG ratios. (H) Representative ROS flow-cytometry histograms and mean fluorescence intensity. Quantitative analyzes used n = 3 independent biological replicates and are shown as mean ± SEM. Two-group comparisons were performed using two-tailed Student's t-test. * P < 0.05, ** P < 0.01, *** P < 0.001.
Anti Pi3k, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+pi3k/PI3K+p110(alpha)+Antibody/pmc13658529-115-27-28
Average 95 stars, based on 1 article reviews
anti pi3k - by Bioz Stars, 2026-10
95/100 stars
  Buy from Supplier

96
Proteintech anti pi3k antibody
ARHGAP40 associates with RhoA and is accompanied by reduced RhoA activity, <t>PI3K/AKT</t> signaling, and altered glutamine-related/redox measures. (A) Flag-based co-immunoprecipitation showing association of ARHGAP40 with RhoA. (B) Endogenous immunoprecipitation of RhoA followed by immunoblotting for ARHGAP40. (C) RhoA activity assay using a conformation-specific anti-RhoA-GTP antibody, with representative GTP-RhoA and total RhoA blots and quantification of the GTP-RhoA/total RhoA ratio. (D) Representative western blots and densitometric analyzes of p -AKT/AKT and p -PI3K/PI3K. (E) Intracellular glutamate levels. (F) Intracellular glutamine levels. (G) GSH/GSSG ratios. (H) Representative ROS flow-cytometry histograms and mean fluorescence intensity. Quantitative analyzes used n = 3 independent biological replicates and are shown as mean ± SEM. Two-group comparisons were performed using two-tailed Student's t-test. * P < 0.05, ** P < 0.01, *** P < 0.001.
Anti Pi3k Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+pi3k/PI3K+p85+alpha+Antibody/pmc13620287-191-75-77
Average 96 stars, based on 1 article reviews
anti pi3k antibody - by Bioz Stars, 2026-10
96/100 stars
  Buy from Supplier

94
ABclonal Biotechnology anti pi3k p85 alpha
ARHGAP40 associates with RhoA and is accompanied by reduced RhoA activity, <t>PI3K/AKT</t> signaling, and altered glutamine-related/redox measures. (A) Flag-based co-immunoprecipitation showing association of ARHGAP40 with RhoA. (B) Endogenous immunoprecipitation of RhoA followed by immunoblotting for ARHGAP40. (C) RhoA activity assay using a conformation-specific anti-RhoA-GTP antibody, with representative GTP-RhoA and total RhoA blots and quantification of the GTP-RhoA/total RhoA ratio. (D) Representative western blots and densitometric analyzes of p -AKT/AKT and p -PI3K/PI3K. (E) Intracellular glutamate levels. (F) Intracellular glutamine levels. (G) GSH/GSSG ratios. (H) Representative ROS flow-cytometry histograms and mean fluorescence intensity. Quantitative analyzes used n = 3 independent biological replicates and are shown as mean ± SEM. Two-group comparisons were performed using two-tailed Student's t-test. * P < 0.05, ** P < 0.01, *** P < 0.001.
Anti Pi3k P85 Alpha, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+pi3k/PI3+Kinase+p85+alpha+Rabbit+pAb/10__1038_slash_s42003___026___10967___6-390-25-30
Average 94 stars, based on 1 article reviews
anti pi3k p85 alpha - by Bioz Stars, 2026-10
94/100 stars
  Buy from Supplier

86
Affinity Biosciences anti p pi3k
ARHGAP40 associates with RhoA and is accompanied by reduced RhoA activity, <t>PI3K/AKT</t> signaling, and altered glutamine-related/redox measures. (A) Flag-based co-immunoprecipitation showing association of ARHGAP40 with RhoA. (B) Endogenous immunoprecipitation of RhoA followed by immunoblotting for ARHGAP40. (C) RhoA activity assay using a conformation-specific anti-RhoA-GTP antibody, with representative GTP-RhoA and total RhoA blots and quantification of the GTP-RhoA/total RhoA ratio. (D) Representative western blots and densitometric analyzes of p -AKT/AKT and p -PI3K/PI3K. (E) Intracellular glutamate levels. (F) Intracellular glutamine levels. (G) GSH/GSSG ratios. (H) Representative ROS flow-cytometry histograms and mean fluorescence intensity. Quantitative analyzes used n = 3 independent biological replicates and are shown as mean ± SEM. Two-group comparisons were performed using two-tailed Student's t-test. * P < 0.05, ** P < 0.01, *** P < 0.001.
Anti P Pi3k, supplied by Affinity Biosciences, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+pi3k/anti+pi3k/pmc13251493-131-18-21
Average 86 stars, based on 1 article reviews
anti p pi3k - by Bioz Stars, 2026-10
86/100 stars
  Buy from Supplier

Image Search Results


ARHGAP40 associates with RhoA and is accompanied by reduced RhoA activity, PI3K/AKT signaling, and altered glutamine-related/redox measures. (A) Flag-based co-immunoprecipitation showing association of ARHGAP40 with RhoA. (B) Endogenous immunoprecipitation of RhoA followed by immunoblotting for ARHGAP40. (C) RhoA activity assay using a conformation-specific anti-RhoA-GTP antibody, with representative GTP-RhoA and total RhoA blots and quantification of the GTP-RhoA/total RhoA ratio. (D) Representative western blots and densitometric analyzes of p -AKT/AKT and p -PI3K/PI3K. (E) Intracellular glutamate levels. (F) Intracellular glutamine levels. (G) GSH/GSSG ratios. (H) Representative ROS flow-cytometry histograms and mean fluorescence intensity. Quantitative analyzes used n = 3 independent biological replicates and are shown as mean ± SEM. Two-group comparisons were performed using two-tailed Student's t-test. * P < 0.05, ** P < 0.01, *** P < 0.001.

Journal: Cancer Biology & Therapy

Article Title: ARHGAP40 suppresses clear cell renal cell carcinoma progression and is associated with altered glutamine-related metabolism and redox balance

doi: 10.1080/15384047.2026.2746137

Figure Lengend Snippet: ARHGAP40 associates with RhoA and is accompanied by reduced RhoA activity, PI3K/AKT signaling, and altered glutamine-related/redox measures. (A) Flag-based co-immunoprecipitation showing association of ARHGAP40 with RhoA. (B) Endogenous immunoprecipitation of RhoA followed by immunoblotting for ARHGAP40. (C) RhoA activity assay using a conformation-specific anti-RhoA-GTP antibody, with representative GTP-RhoA and total RhoA blots and quantification of the GTP-RhoA/total RhoA ratio. (D) Representative western blots and densitometric analyzes of p -AKT/AKT and p -PI3K/PI3K. (E) Intracellular glutamate levels. (F) Intracellular glutamine levels. (G) GSH/GSSG ratios. (H) Representative ROS flow-cytometry histograms and mean fluorescence intensity. Quantitative analyzes used n = 3 independent biological replicates and are shown as mean ± SEM. Two-group comparisons were performed using two-tailed Student's t-test. * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: Membranes were blocked with 5% non-fat milk in TBST and incubated overnight at 4 °C with primary antibodies: β -actin (CST, 4970, 1:1000), ARHGAP40 (MyBioSource, MBS9215052, 1:1000), AKT (CST, 9272, 1:2000), phospho-AKT Ser473 (CST, 4060, 1:1000), PI3K p85 (CST, 4257, 1:1000), phospho-PI3K p85 Tyr458 (CST, 4228, 1:1000), RhoA (CST, 2117, 1:2000), E-cadherin (CST, 3195, 1:2000), N -cadherin (CST, 4061, 1:2000), and vimentin (CST, 5741, 1:2000).

Techniques: Activity Assay, Co-Immunoprecipitation Assay, Immunoprecipitation, Western Blot, Flow Cytometry, Fluorescence, Two Tailed Test